Study of the regulation of molecular condensates in the nucleus in response to PKA and EPAC1 activity
Scientific-Disciplinary Group
05/BIOS-06 - Physiology
Description
The project aims to elucidate how cAMP signals areperceived and translated in normal and cancer cells, and todefine the mechanisms underlying the formation andfunction of EPAC1 nuclear condensates. The candidate willinvestigate the spatiotemporal dynamics of activation of thecAMP–PKA axis, with particular emphasis on EPAC1localization and its role in nuclear condensate formation.The candidate will employ FRET-based imaging techniquesin cell cultures to monitor cAMP levels and enzymaticactivity in real time, integrating biochemical approachessuch as Western blotting and subcellular fractionation. Theactivity will include the analysis of the signals drivingcondensate formation and their impact on transcriptionalregulation, with the goal of defining new paradigms ofnuclear signalling relevant to physiology and cancer.
Job posting website
Funding body
Università di Pavia
Selection process
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