Study of the regulation of molecular condensates in the nucleus in response to PKA and EPAC1 activity

Position: Research appointment (pre-doc) Institute: Uni. Pavia
Posted on: 24/04/2026 Deadline: 29/05/2026

Scientific-Disciplinary Group

05/BIOS-06 - Physiology

Description

The project aims to elucidate how cAMP signals areperceived and translated in normal and cancer cells, and todefine the mechanisms underlying the formation andfunction of EPAC1 nuclear condensates. The candidate willinvestigate the spatiotemporal dynamics of activation of thecAMP–PKA axis, with particular emphasis on EPAC1localization and its role in nuclear condensate formation.The candidate will employ FRET-based imaging techniquesin cell cultures to monitor cAMP levels and enzymaticactivity in real time, integrating biochemical approachessuch as Western blotting and subcellular fractionation. Theactivity will include the analysis of the signals drivingcondensate formation and their impact on transcriptionalregulation, with the goal of defining new paradigms ofnuclear signalling relevant to physiology and cancer.

Funding body

Università di Pavia

Selection process

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The application for admission to the selection procedure (dated and signed),the documents and publications considered useful for the evaluation must be submitted, under penalty of exclusion, electronically, by visiting the following web page: https://pica.cineca.it/unipv/tipologia/idr selecting the Competition Notice you have chosen. In order to register with the system, you must have an email address. Following the guidelines published in the procedure, the applicant must enter all the information required for the application, enclosing all documents in electronic .PDF format. The selection of candidates is carried out on the basis of qualifications and possible interview (with test of knowledge of the foreign language) (see Art. 1 par. 1 Call for applications). The public call with detailed attendance and selection rules (Art 4 and 6) can be found at: https://pica.cineca.it/unipv/tipologia/idrThe selections are open to candidates, Italian or foreigner, in possession, on the date of the deadline for submitting applications for the admission to the selection, of a master's degree or single-cycle degree, preferably in Biotechnology or Biological Sciences, obtained no more than six years previously are eligible to participate in the selection process. These degrees must be relevant to the subject of the research activity. Further specific assessable qualifications (see Art. 1 par. 1 Call for applications): PhD degree or completion of the final year of a PhDprogram, preferably in the biological or biomedical field. PhDthesis and Master’s degree grade. Experience in cell culturetechniques, cell fractionation and organelles isolation, FRETanalysis, and biochemical methods for protein analysis,including electrophoresis and Western blotting,immunofluorescence, and fluorescence microscopy.Experience in the study of second messengers (particularlycyclic AMP and Ca²⁺).