Development, validation and application of LC-MS/MS and UHPLC-MS/MS methods for steroid profiling within the 11β-HSD system

The research assignment will support the activities of the FIS2-funded project “Reshaping The Landscape of the 11 beta HSD System In Androgen Metabolism Kinetics”, project code FIS-2023-02063, CUP B53C25001010001. The activity will focus on the development, optimisation, validation and application of LC-MS/MS and UHPLC-MS/MS methods for targeted steroid profiling in human biological matrices and experimental models, with particular reference to glucocorticoids, classical and 11-oxygenated androgens, cortisol, cortisone and related metabolites. The appointee will contribute to sample preparation, analytical quality control, quantitative analysis and interpretation of steroidomic data generated within the project