2026_IR_49_DIBIO Notice direct award n. 1 Research Assignments - “Interrogating the impact of fructose-to-acetate conversion for nucleolus organization and pancreatic cancer predisposition”

- in vivo studies on "KC" mouse models fed fructose, with 13C isotope tracing and measurement of acetyl-CoA in the pancreas;- histopathological assessment of tumor burden and grade; culture of pancreatic epithelial cells treated with fructose or acetate;- nucleolar analysis (imaging, sucrose gradient purification, protein acetylation by mass spectrometry and western blot);- polyribosome profiling to define the translatome;- analysis of nucleolar morphology in human surgical specimens.